Part of the TeachMe Series
star star star star star
based on 6 ratings

Original Author(s): Oliver Jones and Chloe Webster
Last updated: 23rd January 2022
Revisions: 13

Original Author(s): Oliver Jones and Chloe Webster
Last updated: 23rd January 2022
Revisions: 13

format_list_bulletedContents add remove

An amniocentesis is a procedure used to sample a small amount of amniotic fluid from around the fetus. It can be performed from 15 weeks’ gestation onwards.

It is performed for similar reasons to chorionic villus sampling (CVS), which is carried out from 10 -13+6 weeks’ gestation. It is not the fluid itself which is of use, but the fetal cells that are present in the fluid.

In this article, we shall look at the procedure, indications and complications of amniocentesis.

Fig 1 - Amniocentesis, used to sample amniotic fluid

Fig 1 – Amniocentesis, used to sample amniotic fluid


First, a local anaesthetic is used to decrease pain for the mother.

Under ultrasound guidance, a needle is passed through the mother’s abdominal wall and into the amniotic sac. The needle should not pass through the placenta unless absolutely necessary.

A small amount of amniotic fluid is taken, and fetal cells are sent for karyotyping and/or PCR.

Note: Rhesus-negative women will need Anti-D immunoglobulin after the test.


The most common indication for amniocentesis is a “high risk” result from a first trimester screening test, or a previous pregnancy affected by a genetic condition.

The fetal cells that are obtained can be tested for various chromosomal conditions, such as Down’s syndrome, Edward’s syndrome and/or Patau’s syndrome.

Therefore, the intended benefit of this procedure is diagnosis or exclusion of particular genetic diseases.


The potential complications of an amniocentesis are:

  • 0.5% risk of miscarriage (RCOG). The risk is higher in a multiple pregnancy
  • False reassurance – a “normal” result may make women feel their baby will be born completely healthy when this may not be the case.
  • Risk of infection, as with most surgical procedures
  • Pain from the procedure
  • Rhesus sensitisation (see here)
  • Increased risk of club foot


The main alternative to amniocentesis is chorionic villus sampling. This is also an invasive procedure, with its own risks. It can come to the same conclusions as amniocentesis and is typically performed earlier in the pregnancy.

Nuchal translucency (measured on ultrasound) and blood tests can give the woman some information regarding the risk of Down’s syndrome, but cannot provide definitive diagnosis or reassurance.